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pstat1 tyr 701 58d6 cell signaling  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc pstat1 tyr 701 58d6 cell signaling
    Pstat1 Tyr 701 58d6 Cell Signaling, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1536 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pstat1 tyr 701 58d6 cell signaling/product/Cell Signaling Technology Inc
    Average 98 stars, based on 1536 article reviews
    pstat1 tyr 701 58d6 cell signaling - by Bioz Stars, 2026-02
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    pstat1 tyr 701 58d6 cell signaling  (Cell Signaling Technology Inc)
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    Immortalised primary bovine fibroblasts were pre-treated for 24 h with increasing concentrations of type I IFN prior to a low MOI (0.001 PFU/cell) infection. a , Western blotting for interferon-stimulated genes <t>pSTAT1,</t> Mx1, and RSAD2 at 24 h post IFN-treatment. b , virus in the supernatant of infected cells were titrated by plaque assay on MDCK cells at indicated timepoint post-infection. Data are from three biological repeats and plotted are the titres from each experiment as mean +/− s.d. Data were log-transformed and the statistical significances between IFN treatmented and untreated viruses within each group were measured by two-way ANOVA with Dunnett’s multiple comparisons tests (α = 0.05). P values in bold indicate statistical significance. c and d , Udder slices infected with r-Tx/37/(H5N1) with 10 6 genome copies/slice of tissue. Staining for NP in the duct of udder slices as brown signal in the epithelium and cells detaching from the epithelium. Mx-1 signal is located in the cytoplasm of duct epithelium of udder slices. Confocal images of udder slices with staining for NP and Mx-1 show little colocalization of the signal (merged) in the duct epithelium of infected udder slices. Bars, 100 μm ( c ) and 20 μm ( d ).
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    Immortalised primary bovine fibroblasts were pre-treated for 24 h with increasing concentrations of type I IFN prior to a low MOI (0.001 PFU/cell) infection. a , Western blotting for interferon-stimulated genes <t>pSTAT1,</t> Mx1, and RSAD2 at 24 h post IFN-treatment. b , virus in the supernatant of infected cells were titrated by plaque assay on MDCK cells at indicated timepoint post-infection. Data are from three biological repeats and plotted are the titres from each experiment as mean +/− s.d. Data were log-transformed and the statistical significances between IFN treatmented and untreated viruses within each group were measured by two-way ANOVA with Dunnett’s multiple comparisons tests (α = 0.05). P values in bold indicate statistical significance. c and d , Udder slices infected with r-Tx/37/(H5N1) with 10 6 genome copies/slice of tissue. Staining for NP in the duct of udder slices as brown signal in the epithelium and cells detaching from the epithelium. Mx-1 signal is located in the cytoplasm of duct epithelium of udder slices. Confocal images of udder slices with staining for NP and Mx-1 show little colocalization of the signal (merged) in the duct epithelium of infected udder slices. Bars, 100 μm ( c ) and 20 μm ( d ).
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    Immortalised primary bovine fibroblasts were pre-treated for 24 h with increasing concentrations of type I IFN prior to a low MOI (0.001 PFU/cell) infection. a , Western blotting for interferon-stimulated genes <t>pSTAT1,</t> Mx1, and RSAD2 at 24 h post IFN-treatment. b , virus in the supernatant of infected cells were titrated by plaque assay on MDCK cells at indicated timepoint post-infection. Data are from three biological repeats and plotted are the titres from each experiment as mean +/− s.d. Data were log-transformed and the statistical significances between IFN treatmented and untreated viruses within each group were measured by two-way ANOVA with Dunnett’s multiple comparisons tests (α = 0.05). P values in bold indicate statistical significance. c and d , Udder slices infected with r-Tx/37/(H5N1) with 10 6 genome copies/slice of tissue. Staining for NP in the duct of udder slices as brown signal in the epithelium and cells detaching from the epithelium. Mx-1 signal is located in the cytoplasm of duct epithelium of udder slices. Confocal images of udder slices with staining for NP and Mx-1 show little colocalization of the signal (merged) in the duct epithelium of infected udder slices. Bars, 100 μm ( c ) and 20 μm ( d ).
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    Immortalised primary bovine fibroblasts were pre-treated for 24 h with increasing concentrations of type I IFN prior to a low MOI (0.001 PFU/cell) infection. a , Western blotting for interferon-stimulated genes <t>pSTAT1,</t> Mx1, and RSAD2 at 24 h post IFN-treatment. b , virus in the supernatant of infected cells were titrated by plaque assay on MDCK cells at indicated timepoint post-infection. Data are from three biological repeats and plotted are the titres from each experiment as mean +/− s.d. Data were log-transformed and the statistical significances between IFN treatmented and untreated viruses within each group were measured by two-way ANOVA with Dunnett’s multiple comparisons tests (α = 0.05). P values in bold indicate statistical significance. c and d , Udder slices infected with r-Tx/37/(H5N1) with 10 6 genome copies/slice of tissue. Staining for NP in the duct of udder slices as brown signal in the epithelium and cells detaching from the epithelium. Mx-1 signal is located in the cytoplasm of duct epithelium of udder slices. Confocal images of udder slices with staining for NP and Mx-1 show little colocalization of the signal (merged) in the duct epithelium of infected udder slices. Bars, 100 μm ( c ) and 20 μm ( d ).
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    Immortalised primary bovine fibroblasts were pre-treated for 24 h with increasing concentrations of type I IFN prior to a low MOI (0.001 PFU/cell) infection. a , Western blotting for interferon-stimulated genes <t>pSTAT1,</t> Mx1, and RSAD2 at 24 h post IFN-treatment. b , virus in the supernatant of infected cells were titrated by plaque assay on MDCK cells at indicated timepoint post-infection. Data are from three biological repeats and plotted are the titres from each experiment as mean +/− s.d. Data were log-transformed and the statistical significances between IFN treatmented and untreated viruses within each group were measured by two-way ANOVA with Dunnett’s multiple comparisons tests (α = 0.05). P values in bold indicate statistical significance. c and d , Udder slices infected with r-Tx/37/(H5N1) with 10 6 genome copies/slice of tissue. Staining for NP in the duct of udder slices as brown signal in the epithelium and cells detaching from the epithelium. Mx-1 signal is located in the cytoplasm of duct epithelium of udder slices. Confocal images of udder slices with staining for NP and Mx-1 show little colocalization of the signal (merged) in the duct epithelium of infected udder slices. Bars, 100 μm ( c ) and 20 μm ( d ).
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    Immortalised primary bovine fibroblasts were pre-treated for 24 h with increasing concentrations of type I IFN prior to a low MOI (0.001 PFU/cell) infection. a , Western blotting for interferon-stimulated genes pSTAT1, Mx1, and RSAD2 at 24 h post IFN-treatment. b , virus in the supernatant of infected cells were titrated by plaque assay on MDCK cells at indicated timepoint post-infection. Data are from three biological repeats and plotted are the titres from each experiment as mean +/− s.d. Data were log-transformed and the statistical significances between IFN treatmented and untreated viruses within each group were measured by two-way ANOVA with Dunnett’s multiple comparisons tests (α = 0.05). P values in bold indicate statistical significance. c and d , Udder slices infected with r-Tx/37/(H5N1) with 10 6 genome copies/slice of tissue. Staining for NP in the duct of udder slices as brown signal in the epithelium and cells detaching from the epithelium. Mx-1 signal is located in the cytoplasm of duct epithelium of udder slices. Confocal images of udder slices with staining for NP and Mx-1 show little colocalization of the signal (merged) in the duct epithelium of infected udder slices. Bars, 100 μm ( c ) and 20 μm ( d ).

    Journal: bioRxiv

    Article Title: POLYGENIC DETERMINANTS OF H5N1 ADAPTATION TO BOVINE CELLS

    doi: 10.1101/2024.11.29.626120

    Figure Lengend Snippet: Immortalised primary bovine fibroblasts were pre-treated for 24 h with increasing concentrations of type I IFN prior to a low MOI (0.001 PFU/cell) infection. a , Western blotting for interferon-stimulated genes pSTAT1, Mx1, and RSAD2 at 24 h post IFN-treatment. b , virus in the supernatant of infected cells were titrated by plaque assay on MDCK cells at indicated timepoint post-infection. Data are from three biological repeats and plotted are the titres from each experiment as mean +/− s.d. Data were log-transformed and the statistical significances between IFN treatmented and untreated viruses within each group were measured by two-way ANOVA with Dunnett’s multiple comparisons tests (α = 0.05). P values in bold indicate statistical significance. c and d , Udder slices infected with r-Tx/37/(H5N1) with 10 6 genome copies/slice of tissue. Staining for NP in the duct of udder slices as brown signal in the epithelium and cells detaching from the epithelium. Mx-1 signal is located in the cytoplasm of duct epithelium of udder slices. Confocal images of udder slices with staining for NP and Mx-1 show little colocalization of the signal (merged) in the duct epithelium of infected udder slices. Bars, 100 μm ( c ) and 20 μm ( d ).

    Article Snippet: The primary antibodies used in this study are: pSTAT1 (Tyr701) - Cell Signaling (9167S), RSAD2 - Proteintech (28089-1-AP), GAPDH - Cell Signaling (2118S).

    Techniques: Infection, Western Blot, Virus, Plaque Assay, Transformation Assay, Staining